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Seahorse Metabolic Analyzers – Measuring Cell Metabolism

Seahorse Metabolic Analyzers simultaneously measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) at intervals of approximately 2-5 minutes. OCR is an indicator of mitochondrial respiration, and ECAR is predominately the result of glycolysis. Cellular oxygen consumption (respiration) and proton excretion (glycolysis) causes rapid, easily measurable changes to the concentrations of dissolved oxygen and free protons in a ‘transient microchamber’ which are measured every few seconds by solid state sensor probes residing 200 microns above the cell monolayer. The instrument continues to measure the concentrations until the rate of change is linear and then calculates the slope to determine OCR and ECAR, respectively.

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